Screening of grapes and wine for azoxystrobin, kresoxim-methyl and trifloxystrobin fungicides by HPLC with diode array detection

摘要

The Quinone outside Inhibitors (QoI) are one of the most important and recent fungicide groups used in viticulture and alsoallowed by Integrated Pest Management. Azoxystrobin, kresoxim-methyl and trifloxystrobin are the main active ingredientsfor treating downy and powdery mildews that can be present in grapes and wines. In this paper, a method is reported for theanalysis of these three QoI-fungicides in grapes and wine. After liquid–liquid extraction and a clean-up on commercial silicacartridges, analysis was by isocratic HPLC with diode array detection (DAD) with a run time of 13 min. Confirmation was bysolid-phase micro-extraction (SPME), followed by GC/MS determination. The main validation parameters for the threecompounds in grapes and wine were a limit of detection up to 0.073mg kg-1, a precision not exceeding 10.0% and anaverage recovery of 93% ±38.